You can back up your USB drive by creating a saved image. You can then take that saved image and clone multiple USB sticks. This guide shows you how to create an image of your USB drive using Windows 10.
Don’t follow this guide if you’re simply copying files from a USB stick. Take the usual drag-and-drop method in File Explorer to transfer files to and from the USB stick.
This guide targets users who need to fully back up or clone a USB stick, such as a USB boot drive.
The difference here is that you simply can’t drag-and-drop its contents to another USB drive.
You need the drive’s master boot record and partition tables too.
Even if the source USB drive isn’t bootable, you still need to make a clone if it has more than one partition.